Because we were interested in comparing fundamental differences in how each model predicted BOLD activity across conditions rather than trial-wise or subject-wise predictions, simulations were performed using the best fit parameters from the behavioral experiment rather than performing separate fits to behavior from the scanning session.
The area of interest is then tessellated into grid cells [12] with the same size as the plots [13,14] and the developed models are used to provide cell-wise predictions of AGB.
However, since the PLS model in this study was based on the preprocessed mean spectra from the entire salmonid pieces, the accuracy of this pixel-wise predictions remains to be validated properly in a further study.
Because of the extensive variation within each of the taxa studied (Fig. 3), despite being collected within a relatively small area (Table 1), taxon-wise predictions about germination, as fractions, are not possible.
The authors have done extra work to apply their method to the Nagano et al. 2012 paper, but I still do not see what new biological insight is gained from the cluster method over gene-wise predictions.
The area of interest is then tessellated into grid cells [ 12] with the same size as the plots [ 13, 14] and the developed models are used to provide cell-wise predictions of AGB.
The pixel-wise prediction is color-coded according to the amount of astaxanthin predicted in each pixel, so that pixels with high values of astaxanthin appears red, while low value astaxanthin pixels appear blue.
The pixel-wise prediction can therefore be used to estimate the astaxanthin distribution in the fillet.
The calibrated PLS model makes it possible to predict the astaxanthin concentration in each pixel of the image - a so called pixel-wise prediction.
The final geographical projections represent the mean point-wise prediction over ten model runs.
In this case, the Micromonospora sp. L5 genome served as the reference for the pair-wise prediction of orthologs in 27 representative Actinobacterial genomes, including both Salinispora genomes.
