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In the defence cuts of that winter, HMS Endurance, the navy's "trip wire" vessel in the region, was withdrawn, leaving just 50 squaddies at Port Stanley - who duly surrendered when faced with overwhelming odds.
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For isometric wire myography, vessel segments from wild type mice aged 10 16 weeks (12.9 ± 0.7 weeks) and TLR4−/− mice aged 11 16 weeks (13.0 ± 0.7 weeks) were used.
For short-term exposures, aortic rings were mounted on intra-luminal wires in a small vessel wire myograph [19,20] containing DMEM without l-Arg (37 °C, continuously perfused with 95%O2: 5% CO2) [14].
Finally, small intraluminal details such as thrombus, stent malapposition, guide-wires and vessel wall dissection were visualized by 3D IV-OCT and compared to conventional 2D images.
Arterial segments (1.0 to 1.5 mm long) were mounted in a small vessel wire myograph (Dual Wire Myograph System 410A, DMT A/S, Aarhus, Denmark).
The arteries were then cut into segments (1.28 ± 0.04 mm long) and mounted as ring-shaped preparations in the Mulvany-Halpern style small vessel wire myograph chamber (Dual Wire Myograph System 410A, DMT A/S, Aarhus, Denmark) to determine the vascular reactivity during isometric conditions as described elsewhere [ 33].
As would be expected from the degree of injury, lesions in wire-injured vessels were significantly greater (p<0.0001 by unpaired t-test) than those in ligation-injured vessels.
Different propagation laws, which take into account for negative stress intensity ratio factors R = Kmin/Kmax < 0, are reviewed, because of their high influence on the fatigue life of wire-wound vessels.
One such instrument has a roller block that couples the front of the instrument to a wire from the vessel.
An operator then positioned a thin wire along the vessel edge which allowed for computer calculation of diameter [ 16].
Rings were mounted on a small vessel wire myograph (Danish Myotech), placed under a resting tension of 1 g and allowed to equilibrate.
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