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Importantly, due to their large size most of the aggregates will not be internalized, and most of the internalized fluorescence corresponds to monomeric species, as confirmed by single-molecule photobleaching experiments (data not shown).
Notably, although high-molecular weight contaminants remain in our samples, they are unlikely to be an issue for internalization by electroporation as they will not be internalized as efficiently as our protein.
This methodology is effective because it is expected that the absence of the transporter in the mutant strain makes this strain resistant to the effects of the toxic substrate given that the substrate will not be internalized by the bacterium.
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Furthermore, cytoplasmic staining was ignored as imaging antibodies will not be easily internalized and will have to bind to receptors on the outside of the cancer cells.
Since NIRF antibodies will not be easily internalized, intracellular molecular targets relevant for optical detection of breast cancer have so far been ignored.
It may seem self-evident, but most children will not be consciously aware of the fact that they are talking to themselves, particularly once it is internalized.
But its benefits will not be fully realized unless governments take steps to empower the forces of change, ensure that the massive positive externalities are internalized and minimize the negative impacts.
Individuals internalize the egalitarian ethos and are guided by it in their economic choices, and hence will not be disposed to bargain hard for extra pay and benefits in exchange for making efficient contributions to the economy.
The more decentralized the bargaining, the less wage externalities will be internalized.
Value-driven behavior that is rewarded with social praise and validation will be internalized.
"Faith," he said, "has to be internalized with a relationship with God, and outside circumstances such as an attack on the World Trade Center may move us to action, but it will not necessarily change us on the inside.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com