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The free-floating sections at 30 µm will be stained for BDNF, p-CREB and p75NTR as described previously[12], [35].The sections were examined under AX-70 fluorescence microscope.
For determination of fiber type, serial sections will be stained for myosin ATPase after preincubation at pH of 4.37, 4.6, and 10.4, to identify types I, IIa, and IIb muscle fibers[ 35].
Your hands will be stained for days!
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The question now, he adds, is whether her record will be stained by a victory for Bashar Assad in Syria.Still, even her critics pay tribute to her high intelligence and astonishing work rate.
Samples will be stained using immunohistochemical techniques for different markers of immune response and dendritic cells.
After culture, 100 μL of sample will be stained with the following conjugated fluorochromes for 15 min at room temperature (CD3-FITC; CD4-Per-CP; CD25-APC; CD134-PE, BD Biosciences, Australia), followed by washing in PBS (1 mL), resuspension in 0.5 mL of 0.5% paraformaldehyde (Sigma-Aldrich, Australia) and then multi-parameter flow cytometric analysis.
Besides, deparaffinized normal tissue sections including heart, liver, spleen, lung, and kidney will be stained with hematoxylin and eosin (H&E) for toxicological analysis.
Napkins will be soaked, and shirts will be stained.
When Thomas imagines a carpet, he thinks about all the ways that carpet will be stained.
With others, it will be stained forever by the same foods.
The embryos were stained for DNA.
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