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Intestinal helminth parasite burdens (Kato-Katz) will be quantified as eggs per gram of faeces.
Amyloid burden will be quantified as SUVR (standard uptake value in cortex relative to cerebellum), a unitless ratio.
Surrogate markers of insulin resistance, HOMA and QUICKI, will be quantified as per our previous work [ 99].
The agreement for the ith patient will be quantified as the relative difference d i between readings (y gi ) and measurement results (y ri ), calculated as d i = 2 y gi − y ri y gi + y ri.
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Arterial calcifications at each arterial site will be quantified qualitatively as absent (0) or present (1).
Th1 (IL-6, TNF), Th2 (IL-4, IL-5, and IL-13), and anti-inflammatory (IL-10 e IL-1ra) cytokines as well as osteopontin will be quantified in duplicate by using commercial cytometric bead array kits (BD Biosciences, USA).
Costs from productivity loss will be quantified using the friction cost method, as recommended in the Netherlands [ 28].
Expression levels of a panel of nine cumulus markers of oocyte competence as aforementioned will be quantified by real-time PCR in cumulus cells removed from one cumulus oocyte complex (COC /patient.
These projections will be quantified using Qvistgaard et al's CF, as conducted by the ultrasound machine's built-in 'colour quantification' software.
Glucose will be quantified using a glucose trinder assay (Stan Bio, Boerne, TX, USA) as per our previous work [ 72].
Bands will be quantified by densitometry (NIH Image® prism).
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