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Indeed mutation R687A decreased the IP accumulation significantly to 25% of the wild type without affecting cAMP signaling (Table 2).
In the wild type control with empty vector (SMG367) the replication pattern was essentially the same as in the wild type without plasmid (Figure 2A and 3A).
Deletion of sigB (ΔsigB) decreased the transcription of ctc, under all conditions, to about half of the level observed for the wild type without light (Figures 2A and 2B).
In fact all of the aminoacyl-tRNA synthetases (IleS, ProS, CysS, GlnS, AspS, ArgS, GltX, AlaS, TrpS, GlyS, Gly Q observed were up-regulated in the Δhfq mutant relative to the wild type without a concurrent change in transcription.
The absolute infection rates (c.f.u./ml lysate) obtained in independent experiments were rather variable, therefore the means and standard deviations of the relative infection rates were used for comparison, setting the values for the wild type without light as hundred percent.
The wt::FRT showed no resistance, similar to the wild type without the FRT site.
Similar(44)
The unimodal MIC distribution indicates that these pathogens are wild types without any acquired resistance [ 25].
The engineered SRK intracellular domain was expressed about 54-fold higher production than wild type SRK, without loss of the kinase activity, suggesting it could be useful for further biochemical and structural studies.
Cosuppression of the hydroxylase gene, however, resulted in the accumulation of limonene (up to 80% of the essential oil compared to about 2% of the oil in wild type plants), without influence on oil yield.
The results indicated that the growth of the mutant strains was comparable to wild type strain without anhydrotetracycline.
Increasing the concentration of PDBu from 100 nM to 1 microM PDBu reduced responsiveness of both mutant and wild type receptors without enhancing phosphorylation of either of the receptor proteins.
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