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Chimeric embryos bearing both wild-type (white) and varying amounts of mutant cells (blue) were examined to determine whether Rx mutant cells could contribute to eye structures in a wild type environment.
DxS-KRAS [http:// www.dxsdiagnostics.com/Content/TheraScreenKRAS.aspx], KRAS-TMGB ([42] and this study), as well as further Q-PCR approaches that have been developed for point mutation assessments [25], [50], may be highly sensitive in detecting rare mutant alleles in a wild type environment.
The effect of Afap1L1a overexpression on filopodia dynamics in PGCs migrating within a wild type environment.
This inducible system can be used in transient experiments to study the behavior of transgene expressing cells in an otherwise wild type environment.
At a glance, the in vivo results show that growth of PAN02 cells was significantly faster in the AT2-KO environment than in the wild type environment, most likely due to a high degree of cell proliferation.
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Clearly, these conditions are not representative of the wild type environments which most of these organisms inhabited as they evolved their gene content.
Mosaic mutant analysis, the study of cellular defects in scattered mutant cells in a wild-type environment, is a powerful approach for identifying critical functions of genes and has been applied extensively to invertebrate model organisms.
When growing in a wild-type environment, kif5aa mutant RGC axons invade the tectal neuropil 1 day later (4 dpf) than wild-type cells.
In control experiments, 90% of wild-type FBM neurons migrated normally from r4 into r6 in a wild-type environment (Fig. 6A).
In reciprocal experiments, the MaSC-enriched subpopulation from Csf1 op / op glands had reduced regenerative capacity in a wild-type environment.
60% of wild-type FBM neurons were similarly capable of migrating into r6 in an nhsl1b morphant or nhsl1b mutant environment, albeit not as well as in a wild-type environment (Fig. 6B).
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