Pollen from field grown, heterozygous (EXPB1/expb1) plants was collected, cleaned, aliquoted into 50, 100, 250 and 500 µL volumes, and sprinkled over virgin silks of true breeding wild type plants (four replicate pollinations per volume, yielding 16 ears).
Tadpoles were obtained by breeding wild type frogs, and were staged according to [ 64].
In LDL receptor knock-out (LDLr−/−) mouse, as well as, in ApoE heterozygous deficient mouse, maternal hypercholesterolemia -induced either by diet or by cross-breeding wild type male mouse with ApoE deficient females- promoted atherosclerosis in aortic arch of the respective offspring [48], [49].
AChE+/− mice (Xie et al. 2000) and wild type (AChE+/+) littermates were maintained by breeding heterozygous males and females (129/Sv genetic background).
By breeding with female C57Bl/6J from an in-house breed wild type mice and SOD1G93A littermates of either sex were generated for the experiments.
5 male Sprague-Dawley rats heterozygous for the G93A SOD1 gene mutation (Emerging Model 2148 Het Male, TACONIC US; Wyeth and Amyotrophic Lateral Sclerosis Association 2002) and 5 wild type females were used for a breeding project at Taconic Breeding Services USAA).
Wild type and knock out mice were generated by breeding of heterozygotic parents.
Wild type and Bmal1-/ mice were generated by breeding of Bmal1+/- males with Bmal1+/- females.
Wild type and knock out mice were generated by breeding of heterozygous parents.
From this line we created, via repeated backcrosses into the non-mutator parental line and then self pollinations, true breeding mutant plants (expb1/expb1) and wild type plants (EXPB1/EXPB1), as well as heterozygous (EXPB1/expb1) plants by crossing the true breeding plants.
