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As demonstrated by the wild type behaviour of both ahk5 mutants to ABA, the AHK5-dependent signalling pathway does not contribute to the ABA response pathway in guard cells.
Therefore three representative breast cancer cell lines were selected as model systems, namely BT474, SKBR3 and HCC1954, of which the latter is known to be trastuzumab resistant due to a PIK3CA mutation, while BT474 exhibits wild type behaviour [ 43].
This important discrepancy indicates that the reaction kinetics used in our model, which have been extracted from the literature, may not provide a faithful representation of the molecular mechanism for the wild type behaviour.
Indeed, some experimental evidence of regulated synthesis for the phosphorelay component Spo0B was not included in the published models, and the reaction kinetics used in our model, which are largely extracted from the literature, may not provide a faithful representation of the molecular mechanism for the wild type behaviour.
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In the S. meliloti-inoculated NF4257 mutant, the MtNIN induction was eliminated, whilst MtN5 showed a wild-type behaviour.
To demonstrate the methodology, we focus on modelling the wild-type behaviour of a selection of 200 circadian genes of the model plant Arabidopsis thaliana.
To demonstrate the methodology and its potential further uses, we focus on modelling the wild-type behaviour of a set of 200 chosen oscillatory expressed genes of the model plant Arabidopsis thaliana.
However, this epistatic effect of env1 deletion was light dependent: both GNA1QLE and GNA3QLE showed the same phenotype as GNA1QL and GNA3QL in darkness, while the Δ env1 mutant shows wild-type behaviour in darkness.
In contrast to the wild-type behaviour however, a distribution of current amplitudes is observed ranging from < 4 pA (closed channels) up to a maximum of 10 pA in both cases.
We use system identification techniques to build accurate models of an example gene regulatory network from time-series data, and proceed to identify the kernel architecture of the network, which is defined as the minimal set of interactions needed to reproduce the wild type temporal behaviour.
While control (co) target cells showed a wild type like growth behaviour of single cells and small homotypic aggregates (Figure 1B, upper left), Wehi 231 HPknockdownswns (kd) formed large cellular clusters (Figure 1B, lower left) phenocopying the general growth of proliferating primary B-cells in vitro.
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