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If any regions of the genome are not represented in the population, additional backcrosses can be made to recover the missing wild segment(s) in the cultivated parent background.
The rapid decrease of wild segment lengths observed between the BC1F1 and BC2F1 generations as well as the good level of recovery of the genetic background of the cultivated variety in BC2F1 generation is of great interest for the genetic mapping of QTLs and the development of Introgression Line ILlibrariesies.
The mapping of a wild segment from A. cardenasii conferring resistance to root-knot nematode [ 67] and the registration of two varieties of peanut 'COAN' [ 68] and 'NemaTAM' [ 69], having identifiable alleles conferring resistance to root-knot nematode transferred from wild species, have also been reported.
The percentage of wild genome in the BC1F1 and BC2F1 generations and its relative diminution between the two generations, the mean size of wild introgression segments per LG and per generation, as well as the distribution of the wild segment lengths were estimated using the genotyping data available for each generation.
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Based on genotype, populations of approximately 100 BILs containing one or more introgressed wild segments may be selected to represent the genome of the wild species donor.
The mean length of the wild segments was 51.8 cM in BC1F1 and 34.8 cM in BC2F1, and the decrease of segment size from BC1F1 to BC2F1 was about -33%.
ILs carrying small wild segments in a constant cultivated genetic background have the advantages of reducing epistatic and linkage drag effects and of improving the resolution of QTL mapping [ 23, 70].
Apart from the study of Burow et al. [ 18], introgression mapping of wild segments in the background of a cultivated variety has been reported in 46 introgression lines originated from the hybridization between A. cardenasii × A. hypogaea [ 66].
The distribution of the lengths of the wild segments in the BC1F1 and the BC2F1 generations was calculated for 14 LGs having a length comprised between 75 and 145.2 cM.
We anticipate the use of field evaluations later in our research within a backcrossing scheme, when advanced lines with wild segments have similar architecture to the cultivated recurrent parental, then, the occurrence of several diseases will be evaluated concomitantly.
To select a subset of BC1F1 and BC2F1 lines providing an optimal coverage of donor genome into the recurrent background, we imposed a target length of the introgressed wild segments of 20 cM, an overlapping of adjacent segments for a given LG and the best possible return to the background of the cultivated variety.
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