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KO mice had a significantly greater width of staining as compared with controls (0.37±0.01 vs 0.86±0.03, n=5, P<0.0001).
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As a categorical assay, IHC has a dynamic range restricted to a limited number (normally 3 to 4) of band widths of staining intensity.
The width of crypts stained with periodic acid-Shiff base, Alcian blue, pH 2.5 (PAS/AB) was used to estimate the amount of mucus accumulation as previously described [ 10].
We estimated mucus accumulation morphometrically by the width of PAS/AB stained crypts, and unexpectedly, the crypt width was significantly increased in lubiprostone treated CF mice as compared to control CF mice.
Quantification of convergence and extension cell-movement defects by analysis of the ratio of the width of krox20 staining and the length from somite-1 to somite-8 was done using ImageJ software as described before (van Eekelen et al., 2010).
The width of lighter purple staining with aldehyde-fuchsin demonstrated wider interspaces in the anterior fiber zone (Fig. 3Ca) than the posterior zone (Fig. 3Cc), suggesting differences in flexibility.
On the fluorescent micrographs the apparent width of the microtubule staining at the periphery at the limit of resolution was around 500 nm.
We quantified this difference by measuring the maximum width of α-bungarotoxin staining in each sample.
After staining, the length and width of the pelvic girdle was measured using callipers.
We consistently observed strong surface staining with SUR1 antibodies (Fig 7B) whereas SUR2 antibodies diffusely stain throughout the width of the cell in a sarcomeric repeating pattern (Fig 7C).
The width of the inflorescence meristem was determined on tissue sections stained with toluidine blue.
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