There was a general trend toward significant decrease in head length, width, area and perimeter of air-dried sperm compared with fresh sperm.
The sea surface temperature is decreased to 6 °C with an approximately 350 km long and 100 km width area in the Arabian Sea after the cyclone passage.
In the first experiment, ASMA was performed on heterospermic pooled samples (fresh, post-cooling after equilibration with the glycerol preservation solution and post-thawing): length, width, area and perimeter were measured.
The purpose of PCA is to derive a small number of linear combinations (principal components) from a set of variables (length, width, area and perimeter), that retain as much of the information in the original variables as possible.
Automated sperm morphology analysis (ASMA) was performed using two image analysis programs to determine the sperm measurements: head size (length, width, area and perimeter), shape (ellipticity, rugosity, elongation and regularity) and tail length.
1. Image grayscale moments: The moments of the T-F binary-segmented image denoted ρ'[n, k] can be used to compute shape features, such as height, width, area, perimeter, compactness, centroid, etc.
For the Western blot analysis, the whole cornea tissue including limbal (about 0.5 mm width) area was carefully dissected and Western blotting was conducted as described below.
In part this is because the analysis is based on only a few measures – lamellar width, area, and smoothness.
A corresponding cell scraper was used to scratch the cells from the specific 6 mm width area in the center).
In such cases, spheroid width, area, or total count of nuclei may not demonstrate expected concentration-dependent responses.
