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Coiled coils are widespread protein domains comprising α-helices wound around each other in a regular fashion.
And in so doing they managed to discover 33 new proteins that bind calmodulin--a widespread protein involved in calcium sensing--and 52 proteins that bind phosphotidylinositides, cell membrane proteins involved in growth, differentiation, and cytoskeletal rearrangements.
It was discovered over time by picking up a thread that began with finding a new widespread protein in our bodies that fits lock-and-key with a major cannabinoid in cannabis, and following that thread until it revealed a massive tapestry of DNA-encoded molecules working in unison, the endocannabinoid signalling system.
When elevated temperature causes widespread protein aggregation, Hsp104 becomes critical for survival by disaggregating proteins in a reaction dependent upon Hsp70 and Hsp40 [20].
In fact, targeting a protein with widespread protein:protein interactions, such as CD81, might explain how MARCH proteins down-regulate such a variety of substrates, with no sequence similarity.
Lower VEGF and osteopontin gradient indicate more widespread protein expression.
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And, finally T12b comprised 217 widespread proteins and 95,528 positions.
First, a well-sampled tree focusing on the 21 species from the Saccharomycotina group was built from a concatenated alignment of 1,137 widespread proteins.
Combinations that did not contained the species S. cerevisiae and did not provided a set of at least 30 widespread proteins for the concatenation were discarded.
To achieve this, we built a concatenated alignment of 69 widespread proteins that were present in at least 58 of the 60 species used and displayed one to one orthology relationships (see Material and Methods).
To assess the extent of congruence between trees based on concatenated alignments and individual phylogenies, we compare the topology of phylogenies of genes encoded in the yeast genome with fungal species trees reconstructed from the concatenated alignments of widespread proteins present across different sets of fungal species.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com