In conclusion, although IES are clearly depleted from exons on a genome-wide scale, expression of an IES-containing mRNA provides a previously unrecognized mechanism for increasing mRNA complexity in a developmentally regulated manner.
Since mRNA levels can nowadays easily be measured on a genome-wide scale, expression profiling has become a first method of choice for assessing the molecular response to experimental perturbation (the molecular phenotype).
These observations suggest that zebrafish embryos can be used as a tool to study the mechanism of histone modification in the context of development, and demonstrate the strength of a wide-scale expression survey to identify the master epigenetic regulator genes.
This is due to the fact that at the genome-wide scale of gene expression, relevant biological differences may be modest or even negligible relative to the noise.
It matters on a wider scale too.
But could the approach work on a wider scale?
These two results suggest that the first three points of each time series (representing the first 12 hours) can be used to identify genes whose expression changes significantly in the early stages of differentiation, before wide scale alterations of gene expression.
An obvious function is the wide scale regulation of gene expression by the targeted degradation of transcript levels.
For additional information please visit the tool website: http://www.agilent.com/labs/research/compbio/enviz/ Contact: [email protected] The recent emergence of novel high-throughput technologies enables the quantification of different types of biological features in a genome-wide scale (e.g. mRNA expression levels, miRNA expression levels and DNA copy numbers).
Although several studies have explored gene expression during flower development in the model plant Arabidopsis thaliana on a genome-wide scale, a continuous series of expression data from the earliest floral stages until maturation has been lacking.
