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The integration of RNA-seq with Augustus and Maker gene discovery did not only increase the number of protein-coding genes, but also improved the predicted gene structures, evidenced by wider alignment coverage (Additional file 7: Figure S3C) and greater percent identity when aligned to the proteomes of other ascomycetes (Additional file 7: Figure S3D).
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For each HapMap SNP, using the human-chimp genome-wide alignment files provided by the UCSC Genome Browser, we defined the human derived allele as the allele that differs from the chimpanzee allele at the same locus.
The bootstrap values generated from a seed-plant-wide alignment of the four coding regions using the 'fast bootstrap' option in PAUP* [19], generally agreed well with values generated using this gene combination for each genus considered separately.
Thus, conserved structure search methods make use of readily available data sets: RNAz makes use of conserved structure databases covering only a small portion of the genome [10], MSARI needs alignments of conserved sequences from as much as 10 different species [8] and EvoFold needs a costly genome-wide alignment of several genome sequences [9].
Conserved regions were identified using a grass family-wide alignment of plastomes.
Recently, using a genome-wide alignment of two Orzya species var.
Many conserved secondary structures (CSSs) were identified in the human genome by using an eight-way genome-wide alignment.
Searching similar pairs from a string pool is a fundamental process of de novo genome assembly, genome-wide alignment and other important analyses.
MDL partitioning can be refined in many ways but is a promising first step in the analysis of genome-wide alignment.
The PEG119 shared 37% similarity with PEG274, while PEG79 shared 27%and3535% with the other two in pair-wide alignment of amino acid sequences.
CSSs were identified by using a human-referenced eight-way genome-wide alignment, but the evolutionary variation of the elements in human populations is unknown.
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