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The most widely used expression system for CHO cells has been the glutamine synthetase (GS) system, which was pioneered by Lonza.
Escherichia coli (E. coli) is the most widely used expression system for the production of recombinant proteins for structural and functional studies.
Therapeutic glycoprotein production in the widely used expression host Pichia pastoris is hampered by the differences in the protein-linked carbohydrate biosynthesis between this yeast and the target organisms such as man.
This article describes the basic biology of the baculovirus and emphasizes the key aspects of its life cycle, which have led to its development as one of the most widely used expression systems today.
Local grid refinement, it is a widely used expression for the process of dividing one or several grids in the reservoir model into smaller sized grids allowing enhanced grid definition, which is useful for modeling wells or hydraulic fractures and other complex reservoir structures.
A widely used expression has a credibility that less popular variations of it do not.
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Although expression of recombinant proteins in bacteria is widely used, mammalian expression systems have advantages for the production of mammalian proteins, allowing correct folding or authentic post-translational modifications.
Baculovirus-insect cell expression system has become one of the most widely used eukaryotic expression systems for heterologous protein production in many laboratories.
Although several small RNA species are recommended for normalising miRNA expression in tissues and cell lines (Wong et al, 2007), the widely used RNU6B expression is not correlated with total RNA concentration in stool (Link et al, 2010).
Though 293T cells are widely used for expression of proteins from transfected plasmid vectors, the molecular basis for the high-level expression is yet to be understood.
During the last decade, alphaviruses became widely used for expression of heterologous genetic information and development of recombinant vaccines against a variety of human and animal pathogens.
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