The XL-30 ESEM is equipped various detectors, such as, secondary electron, gaseous secondary electron, wide-field secondary electron detectors, back scattered electron Detectors (GBSD and BSD) for both high vacuum and gaseous environment.
The BAT, a wide-field γ-ray detector, will detect >100 γ-ray bursts per year with a sensitivity 5× that of BATSE.
By analogy to models for direction-selective motion detectors where wide-field optic flow can be deduced by summing output of local elementary motion detectors, we use the term 'elementary small target motion detector' (ESTMD) for this stage.
Wide-field FLIM by the Quadrant Anode detector is a valuable technique to measure the fluorescence decay of fluorophores at very high temporal resolution.
Some models for target discrimination rely on inhibitory feedback of wide-field motion signals to localized motion detectors [11], [12], which may provide an explanation for small target selectivity, but would lead to inhibition by background motion.
The use of wide-field patterns reduces the number of source-detector pairs required for tomographic reconstruction drastically reducing the time of acquisition.
This last model is new to this revision, but we believe it is informative because it moves us further beyond the HRC and is motivated by EM reconstructions that show wide-field inputs to the fly's motion detectors.
Wide-field deconvolution microscopy (WFM) – where a photo-detector like a charge-coupled device (CCD) camera is combined with computational techniques that use a measure of the blurring – the microscope objective point-spread function – to reduce blurring and calculate an image with substantially improved contrast helped reveal even more cellular substructure 7, 8.
The results of the three illumination schemes were then compared to determine the effect of choosing a targeted illumination scheme over a wide-field illumination scheme, as well as a wide area detector (i.e., PMT or APD) over a small area detector (i.e., camera pixel) based on the desired signal distribution.
