First, the age range of the obese and lean participants in this study is wide and the sample size is small.
In the case of the more reliable subsets of phosphoserines observed in several experiments, the subtitution rate to aspartate and glutamate is even higher, and also lies outside the interval of bootstraps that in this case is wider, as the sample size is smaller.
Nevertheless, because non-parametric tests trade-off power for increased robustness and wider applicability, the sample size for most RNAi screening studies will be too small to enable conclusions from non-parametric tests with the same degree of confidence as from parametric tests.
Note also that the confidence intervals of the thresholds in the individual studies were rather wide, depending on the sample size.
However, this mutation is not present in all VISA strains, and therefore, the phenotype must be caused by other mutations that are not detectable by association at genome-wide significance levels given the sample size of strains available.
The t values will always be larger, leading to wider confidence intervals, but, as the sample size becomes larger, the t values get closer to the corresponding values from a normal distribution.
Findings suggest similar agreement in mean values but wider levels of agreement; however, the sample size was substantially reduced.
Therefore, financial constraints that limited the sensitivity and breadth of the genome-wide scan, as well as the sample size, were possible reasons for the lack of significant findings in Stages 1 and 2. Indeed, power relies on the design employed and the sample sizes.
Although the point estimates are wide apart, the relative smallness of the sample size means that the difference is only borderline significant from the statistical point of view.
The sample size and wide variance in PEI scores created margins of error such that no conclusions can be made regarding differences between the means.
