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The advancement of genome wide techniques has not only allowed the generalisation of findings from well-studied, isolated examples, but has also helped uncover hitherto unnoticed biological phenomena.
Furthermore, due to the T2C design, a better coverage and resolution of the locus is obtained when compared to other genome wide techniques (like Hi-C and 3C with its derivatives) using a 6 bp cutter as first restriction enzyme.
Another genome-wide technique that can be used to identify NMD targets is analysis of mRNA decay rates, with the assumption that direct NMD targets will show increased stability upon inhibition of NMD.
Conserved consensus motifs have been predicted for transcription factor binding across the human genome, and empirical transcription factor binding sites (TFBS) have been determined biologically using the genome-wide technique which couples chromatin immunoprecipitation and high throughput sequencing (ChIP-seq).
They relied on a genome-wide technique called parallel analysis of RNA ends (PARE) or degradome sequencing, where mRNA fragments bearing 5′ monophosphate termini are specifically selected by adaptor ligation and short sequence tags representing their 5′ ends are generated and analyzed by high-throughput sequencing.
In recent years, genome-wide techniques (e.g., microarrays) in particular the transcriptomes of miRNAs have been widely used to study the biological function of miRNAs.
The Enigma technology was obsolete, he said, but "the wider techniques that we used for studying German communications are, broadly speaking, the same techniques that are used against terrorism now, so there is no reason why that side of things should be made public It is all still operationally useful".
Recent advances in genome-wide techniques have led to a rapid expansion in our detection and understanding of epigenetic landscapes.
The latest advances in genome-wide techniques challenged the "protein-central dogma" with the discovery of the so-called non-coding RNAs (ncRNAs).
To elucidate the molecular mechanisms of drug resistance investigators have employed several genome-wide techniques, including transcriptome analysis, to identify genes and their associated pathways in developing chemoresistance.
Such regions can currently only be detected by genome-wide techniques such as MeDIP-seq.
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