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Prediction and thermodynamic analysis of secondary structure formation in a genome-wide set of transcripts from Brucella suis 1330 demonstrates that the properties of the target molecule have the potential to strongly influence the rate and extent of hybridization between transcript and tethered oligonucleotide probe in a microarray experiment.
Using a nanostring device, which digitally quantifies a wide set of mRNA transcripts starting from a very low quantity of tissue, we analysed RNA samples obtained from DRGs of mice which received intrathecal injections of 10 pmol of miRNA-1a-3p inhibitor or mismatch inhibitor in vivo every 24 h with in vivo transfection reagent from PID-4 to PID-9.
We identified a genome wide set of the targets of miR-503, −103, and −494 by conducting extensive profiling of RISC associated transcripts and gene expression profiling.
These use an equally wide set of chemicals as substrates.
A wide set of traits may be affected.
These sequences represent the full set of assembled transcripts.
"A wider set of clients is interested".
Preferably, the experiment will cover a wider population and a wider set of sampling conditions.
The wide variation in expression values for the nonannotated set of transcripts likely reflects that many of these transcripts are assembly errors rather than functionally transcribed genes.
We have consolidated existing transcriptomic data for S. mediterranea to generate a high confidence set of transcripts for use in genome wide expression studies.
This reduced set of transcripts yielded 46,657 transcript isoforms, 24,264 unique transcripts and a higher N50 of 1761 (Table 1).
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CEO of Professional Science Editing for Scientists @ prosciediting.com