Given that PLCζ has been detected in tissues and cells other than sperm in non-mammalian species [ 68], it would appear prudent to carry out wider scale expression studies to re-confirm that PLCζ is specifically expressed in human sperm and not any other cell or tissue-type.
In conclusion, although IES are clearly depleted from exons on a genome-wide scale, expression of an IES-containing mRNA provides a previously unrecognized mechanism for increasing mRNA complexity in a developmentally regulated manner.
Since mRNA levels can nowadays easily be measured on a genome-wide scale, expression profiling has become a first method of choice for assessing the molecular response to experimental perturbation (the molecular phenotype).
These observations suggest that zebrafish embryos can be used as a tool to study the mechanism of histone modification in the context of development, and demonstrate the strength of a wide-scale expression survey to identify the master epigenetic regulator genes.
These two results suggest that the first three points of each time series (representing the first 12 hours) can be used to identify genes whose expression changes significantly in the early stages of differentiation, before wide scale alterations of gene expression.
An obvious function is the wide scale regulation of gene expression by the targeted degradation of transcript levels.
While it is possible that Theileria parasites deploy similar mechanisms to manipulate gene expression on a wide scale, our results suggest additional alterations are involved.
Among expression profiling methods, RNA-seq has been a breakthrough to quantify gene expressions on a genome wide scale.
Since an shRNA library carries a large pool of potential siRNAs, such a library makes it possible to knock down gene expression at the genome wide scale.
The identification of ncRNAs has been facilitated by comparative genomics and development of methods to detect RNA expression on a genome wide scale.
Theglobal expression studies are generally used in "Discovery phases"including a wide scale analysis of many housands of peptide sequences thatoriginates from digested proteins.
