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Since we didn't have any specific PCR primer to amplify the whole rDNA cluster, we decided to proceed with a wide restriction enzyme screening using the following enzymes: Taq, Alu, Sma, Ava, HpaI, HpaII, ClaI, SstI, StuI, CfoI, HhaI, MspI, DraI, HincII, XbaI and PstI.
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An EU-wide restriction of Deca-BDE followed in 2008 (curia.europa.eu; case C-14/06 C-14/06
To elucidate the origin of the deletion, we have matched genome-wide restriction fragment length polymorphism (RFLP) data at 179 loci and two other FNPs (qSH1 and Wx) that were also involved in the domestication of japonica rice [13, 16, 24].
In particular, flowering-time genes are likely to have been involved in the domestication process to permit rice to grow in northern areas, although recent breeding steps may conceal older changes that occurred during rice domestication Fig. 2 Genome-wide restriction fragment length polymorphism (RFLP) patterns at 179 loci.
The OM technique is a non-PCR-based method to generate genome-wide restriction enzyme maps, which improves the quality of de novo genome assembly.
Because most TDCPP-specific genotoxicity assays have been negative in vivo (Bloom 1984; Brusick et al. 1980; Cifone 2005; Morales and Matthews 1980), we used a screening-level, genome-wide restriction analysis approach to determine whether the susceptibility of cleavage was associated with adverse effects on zygotic genome methylation.
Companies have warned of wider restrictions, including extending the hosepipe ban to businesses.
Health and environment campaigners concerned about contaminated groundwater now are calling for E.U.-wide restrictions.
Last month the European Commission imposed Europe-wide restrictions on exports of key anaesthetics used to kill prisoners in the US.
But over concerns about the huge decline, EU-wide restrictions were imposed in 2010, limiting the trade to within the European Union only.
While Russia had earlier suggested that the UK was acting prematurely in halting flights to the Red Sea resort over terrorism fears, Vladimir Putin ordered even wider restrictions on Friday, including halting all flights from Cairo.
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