Such recent results should provide encouragement for additional studies using a wider range of iPSC differentiated cell types in both murine and non-human primates and ultimately in clinical studies.
For a wide range of values for cutoffs of sample-level differentiated genes, SLEPR maintains the main biological themes in its analysis results in that all the top ranked terms based on the original 1× MADe cutoff are primarily ranked at the top level (most of the terms still remained at the top level: ranked within the top 10 functional terms and p-value less than or close to 0.01).
There is growing evidence that specific chromatin modifications distinguish stem and differentiated cells in a wide range of tissues.
In this study, we used the array platform GeneChip Human Genome U133 Plus 2.0 (HG-U133 Plus 2.0) to analyse the expression of a wide range of genes (>30 000) in well- and poorly differentiated thyroid tumours and, to correlate, for the first time, gene expression with BRAF, RAS, RET/PTC and PAX8-PPARG alterations.
However, for hESC to be used in a wide range of treatments, it is imperative to minimize immune responses following transplantation of differentiated progeny.
Clearly, although neurons are terminally differentiated cells, they do express a wide range of cell cycle proteins and are known to be capable of replicating their DNA, although no cases of a neuronal cell division have ever been reported.
Similarly, in cell culture, evaluation of tumour spheres that disaggregated into single cell suspensions demonstrated that they are more resistant, compared with their differentiated progeny, to irinotecan across a wide range of irinotecan concentrations.
Survivin is a recently described IAP that is unique for its expression in a wide range of embryonic and foetal tissues but is undetectable in terminally differentiated normal adult tissues.
Hybridization (interbreeding between genetically differentiated lineages) takes place in a very wide range of organisms (Barton and Hewitt 1985, Dowling & Secor 1997, Mallet 2005) and may play an active role in a variety of evolutionary processes ranging from local adaptation to speciation (Stebbins 1959; Arnold 1992; Barton 2001; Rieseberg et al. 2003).
