In addition, there is a growing collection of databases of gene expression for the BXD lines at that provides an additional level of genome wide interrogation [ 9- 11].
Here we report such a methodology (Microarrays-before-Sequencing, Mab-seq), which should also be of interest to investigators using chromatin immunoprecipitations who are working primarily with microarrays, but wish to bank material for later genome-wide interrogations.
These early findings were restricted to only a subset of chromosomes and did not include genome-wide interrogations of interference.
We reasoned that databases providing results from expression arrays or transcriptome-wide interrogations of tissues or body fluids comparing preterm deliveries with similar samples from those at full term would provide a hypothesis-free interrogation.
Very recently, two studies further expanded the application of CRISPR/Cas9 to genome-wide interrogation of gene function through systematic transcriptional perturbation.
A high throughput derivative of 3C, chromosome conformation capture on chip (4C), executes a genome-wide interrogation of interaction partners for a given locus.
Once we had proven that there was intact RNA in plasma and that we could amplify it to a degree which would allow genome-wide interrogation, we used mouse models of acute organ injury to identify differentially expressed genes following injury.
We have performed a genomie-wide interrogation of the brain transcriptome of C57Bl/6 mice at 145 days post inoculation (dpi) intraperitoneally (i.p).
We designed microarrays that represent >80% of the yeast proteome, enabling a nearly genome-wide interrogation of RNA-protein interactions in vitro.
The advent of microarray technology has led to genome-wide interrogation of transcript abundance.
The concept of genome-wide interrogation, nonetheless, can also be applied outside genetic association and case/control setup.
