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Because of the extremely wide detection bandwidth of the optical micro-ring resonator [ 30], we expect that the axial resolution can be significantly improved.
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Thus, the illumination frequencies are limited by the detection bandwidth.
The detection bandwidth is equal to the inverse of the frame exposure time, which is 10 msec for these data.
Again, the −6 dB roll-off point is found to be at ~950 MHz close to the detection bandwidth.
Key to these developments have been improved control over analyte translocation speed, reduced electrical noise, and increased detection bandwidth.
Due to detection bandwidth limitations, the true depth of the −6 dB point might actually be higher.
The detection bandwidth scales inversely with the sampling time, which for a minimum of a 125 ns acquisition time corresponds to 32 MHz of frequency bandwidth about the center frequency in the underlying electronics.
The PLE spectrum taken with 450 460 nm detection bandwidth hints to a p-6P optical absorption resonance peaked at ∼370 nm.
Alexa 488 was excited using an argon laser (488 nm), and the detection bandwidth was set at 493 to 601 nm (expected peak at 520 nm).
The wide emission bandwidth of the NT.LabelFree instrument (330 380 nm) likely prevented SOD1 denaturation being detected by intrinsic fluorescence emission [40].
Apart from wide impedance bandwidth, the observed pattern bandwidth is also similar.
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