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A positive correlation between eIF4G level and translational efficiency of genes whose translation is expected to be more Pab1-dependent supports previous studies showing that Pab1-mediated, poly(A) tail driven stimulation of translation in vitro requires eIF4G [19].
We observed that this motif is preferentially located in the coding DNA sequence (CDS) and to a lesser extent in the 5′UTR of SRSF1 translational targets, when compared with those mRNAs whose translation is unaffected by increased SRSF1 expression (referred to as null [PSR∼0]).
This constraint may result from the requirement to preserve either the translational regulatory elements (uORFs) in 5'UTRs or the functions of the peptides whose translation is associated with uORFs.
Last, the mRNAs whose translation is activated by Xrn1 have an unfavorable translation initiation context.
Despite this progress, mRNAs whose translation is altered in chronic pain conditions remain largely unknown.
Genome-wide transcription and translation analyses were performed to identify mRNAs whose translation is highly sensitive to reduction in DAP5 levels.
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Genome-wide translational profiling in mouse embryonic fibroblasts (MEFs) revealed a subset of mRNAs whose translation was reduced in the Eif4e ki (Ser209Ala) mice (Furic et al., 2010).
The Bodleian has supplied the only surviving annotated copy of the earlier Bishops' Bible, whose translation was used as a foundation for the King James.
John Minford, whose translation was published in 2002, says that after Gordon Gekko, a villainous corporate raider played by Michael Douglas in the film "Wall Street", quoted a line from Sun Tzu ("Every battle is won before it's ever fought"), the book acquired a "mystique" among students of entrepreneurship.Professor Minford says he is mystified by this.
While we expected activation of translation as a function of eIF4E induction, the large number of genes whose translation was repressed after eIF4E induction is surprising.
We focused our attention on newly identified uORF-bearing mRNAs whose translation was refractory to eIF2 inactivation.
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