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Our original series comprised three male CFNS cases (Subjects 3269, 1330 and 4021; Fig.  1B D), from whom we analysed DNA extracted from multiple tissue samples (blood, buccal scrapings and hair roots) because the level of mosaicism might vary depending on the timing and tissue origin of the mutation.

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For those who were unsuitable for, or refused transfer to, commissioned VV centres, and in whom we initiated VV ECMO, we analysed outcomes and organ failure.

Therefore, the patients were divided into two groups: the first group comprised patients from whom we had the opportunity to analyse KLK6 mRNA levels in H&E nodes and the second group comprised patients from whom only H&E nodes were available for mRNA analysis.

In the Cox regression analyses, participants for whom we had complete data on all measures at all intervals preceding death or the end of follow-up (after imputation) were censored at their date of death or at the end of follow-up.

Therefore, to minimize selection bias, we performed a complete case analysis in which we restricted the longitudinal analyses to participants for whom we had adequate data for analysis at both baseline and at least 1 follow-up health examination.

Results obtained when we restricted analyses to births for whom we had gestational age did not appreciably differ from results obtained assuming all births occurred at term and using imprecise estimates of relevant exposure windows.

We recruited 1,919 consenting participants from whom we obtained 1,898 completed surveys for the current analyses.

All statistical analyses were thus performed among the 210 subjects for whom we had genotype information.

We know whom we own.

21 22 23 Missing results were imputed for all variables evaluated for the diagnostic model but not for "pneumonia," as we analysed only participants for whom this diagnostic outcome was known.

We analysed data for 712 subjects of whom 60.5% were Kyushu/Okinawa residents.

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