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Read counts per sample were obtained using HTseq version 0.5.4p1 using "intersection-strict" mode, whereby the whole read must map to a single transcript to be counted.
"The Radio 1 audience has never on the whole read the Radio Times.
If an exact match of a seed s exists, then we extend it to the whole read and find mismatches for the whole read (Algorithm 3, lines 9 11).
Nearly all state-of-the-art sequence aligners start alignment process when whole read data is available.
These books can be digested as a whole, read and enjoyed".
The most important difference between the movie and technology industries lies in each community's very raison d'etre -- movies are rooted in art and personality and technology is driven by increasing the efficiency of transactions (for a good overview of this in the economy as a whole, read: The New York Times', "Economics Made Easy: Think Friction").
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So, the whole reads 'Well fare mi lady'.
The whole reads like a disassembled quilt, one discrete patch neatly neighboring another.
The whole reads were removed if the remaining length was shorter than 20 nucleotides.
Remaining whole reads are then mapped to the reference genome, and then spliced alignments are attempted.
The whole reads were assembled with Newbler (Roche) and validated via the Consed interface (www.phrap.org, last accessed June 17 , 2014.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com