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Confocal images from whole mount of retina were used to calculate the cone cell density (PNA or red/green opsin positive cells) in the superior retinas using software Volocity (Improvision, Waltham, MA).
Whole mount of tissue was sampled from proliferating PEMs (stage 1) and early embryos (stage 2).
A whole mount of a microdissected synovial sheet was stained with an antibody against vimentin and imaged using the confocal microscope (Fig. 1 A ).
As a reliable indicator of in vivo senescence, DNA damage foci defined by phosphorylated H2A.X (γ-H2A.X) was increased in the whole mount of epidermal sheets including SG from p53 TSD /− mice.
DNA damage foci, a reliable indicator of in vivo senescence, which was increased in the whole mount of tail skin from p53 TSD /− mice, was partially rescued by BADGE, specifically in the SG but not in adjacent bulge or hair germ cells.
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After tracer transport, thoracic and abdominal esophagi were removed and prepared as whole mounts of muscle wall without mucosa or submucosa.
Whole mounts of transgenic mammary glands were also stained with Carmine Alum using a standard protocol.
Whole mounting of wild type and agroinfiltrated leaves was carried out as described previously [49].
Whole mounts of wild-type mammary outgrowths were stained with hematoxylin.
We therefore examined the state of histone modifications in label retaining cells (LRC) in whole mounts of mouse tail epidermis (Fig. 2A F) [2A F
The host animals were bred two months later and whole mounts of the glands were analyzed at day 1 of lactation (D1L).
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