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The whole genome size was 372.50 Mb.
Because the whole genome size of spheroid bodies is not experimentally determined so far, it is not known if these modifications have lead to an overall decrease of the spheroid body genome size.
In contrast, B. neritina has features that are more typical of metazoan mitochondrial genomes in general in terms of the number of gene components, whole genome size and A+T content.
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When an average size of 4.4 × 106 bp of bacilli genomic DNA was considered (calculated from whole genome sizes available from the NCBI database), this library theoretically covered the genome size of at least 91 and 302 times (depth of coverage) when the DNA fragments of 3,000 and 10,000 bps, respectively, were taken into account.
The total length of contigs (5.00 Mb) and number of contigs (7589) corresponded to 13.5% of the whole-genome size (37.05 Mb) and 54.0% of the number of predicted ORFs (14 063), respectively (Fig. 1, Table 1).
Linear regressions of LCNS against whole-genome size (C-value) and diploid number of chromosomes (2n) did not demonstrate a discernible correlation (LCNS vs. C-value: R = 0.041; LCNS vs. 2n: R = 0.206).
A recent analysis of whole-genome size in 11 red- and white-blooded notothenioid species confirmed that the evolution of phylogenetically derived notothenioid families, such as the Channichthyidae, might have been accompanied by a genome expansion (Detrich and Amemiya 2010), without further indication, however, of which genes or gene families are involved in such expansion.
Whole-genome sizes were considered in terms of C-value, diploid number of chromosomes, and total sequence length (Gb).
To test the hypothesis that LCNS abundance scaled with other measures of genome length, the numbers and total lengths of LCNS were regressed against mean whole-genome sizes of the species in which the LCNS is shared (Olmo 1976; Tiersch et al. 1989; Peterson et al. 1994; Vinogradov 1998; Gregory 2005; Johnston et al. 2007; Pigozzi 2008).
I.e., we divided counts by profile-specific (whole genome) library size.
There are 19 confirmed prophage regions (only one is intact, i.e., not missing any necessary prophage components); the prophage sequences are sporadically distributed across the whole genome with sizes ranging from 10.3 kb to 35.7 kb; the mean GC content is 60.27%.
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