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The isooctane layer was separated from the aqueous layer into a clean glass tube and the whole extraction procedure was repeated again.
The impact of initial water saturation on gas production can not be ignored in the whole extraction process, especially during the water drainage period.
Two practical extraction techniques including stir bar fabric phase sorptive extraction (stir bar-FPSE) and magnetic stir fabric phase sorptive extraction (magnetic stir-FPSE) have been designed, which allow stirring of fabric phase sorbent during the whole extraction process.
We also performed experiments that only enrollment and test sets were extracted using CRA algorithm, and results show that both approaches gave better results than the baseline of traditional i-vector extraction approach, but the results of whole extraction (development set, enrollment set and test set) are best.
The whole extraction process was repeated three times.
However, the peak areas were much smaller with 133,017, 139,258, and 135,774 AU, because the analytes were diluted to the whole extraction volume plus the void volume of the trap, which remains in the tube after the injection.
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Whole extractions were then subjected to restriction digests with 5 µg of DNA and 25 U of EcoRI in a total of 25 µL.
Individual crypts were isolated by whole mount extraction and underwent RNA extraction and qRT-PCR for analysis of Wnt target gene expression (online supplementary methods).
Many RNA-seq studies currently use composite extractions, and even whole insect extractions, when tissue and structure specific extractions are possible.
Proteins were extracted using a Whole Cell Extraction Kit (EMD Millipore Corporation, Billerica, MA, USA), according to the instructions.
Subsequent experiments used direct whole cell extraction in the high salt (nuclear extraction) buffer.
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