The white nuclei were re-suspended in 40% guanidine hydrochloride.
In the spermatids of ref(2 P od2 mutants, the nebenkern enveloped the white nuclei instead of being adjacent to them, as is the case in the control spermatids.
Using co-immunostaining against fibroblast marker (Vimentin, in red, and 2(i))) and EdU (white nuclei = proliferative cells), we confirmed the abnormal proliferation of adventitial fibroblast cells (Vimentin positive cells) in MMC-exposed rats) compared to control rat).
To calculate the nuclear-to-cytoplasmic ratio at each ROI in the hematoxylin-eosin image, the digital images were segmented by hand and converted to a binary image, with white nuclei and black cytoplasm.
Its nucleus is bright enough to stand out in broad daylight, and by night its foggy milk-white nucleus and bridal-veil tail make for one of the prettiest things humans have ever seen in the sky, and one of the last.
(J) Mean FRET ratios in the cytoplasm (white) or nucleus (grey) in LEDGF/p75D366N, LEDGF/p75BC and LEDGF/p75WT cells infected with HIVIN-mTFP1+IN-mVenus.
(A) Fluorescence intensity of VSV-G pseudotyped HIV complexes (HIVIN-eGFP) in the cytoplasm (white) or nucleus (grey) of cells treated with DMSO, RAL (0.6 μM) or EVG (0.2 μM) during infection.
(I) Mean FRET ratios in the cytoplasm (white) or nucleus (grey) in HeLaP4 cells infected with VSV-G pseudotyped HIVIN-W131A-mTFP1+IN-W131A-mVenus compared to HIVIN-mTFP1+IN-mVenus.
(B) Fluorescence intensity of VSV-G pseudotyped HIVIN-eGFP complexes in the cytoplasm (white) or nucleus (grey) of HeLaP4 WT cells treated with DMSO or LEDGINs (45 μM, CX14442) during infection.
(D) Mean FRET ratios of VSV-G pseudotyped HIVIN-mTFP1+IN-mVenus complexes in the cytoplasm (white) or nucleus (grey) of LEDGF/p75KD cells treated with DMSO or LEDGINs (45 μM, CX14442) during infection.
(F,G) Fluorescence intensity (F) and mean FRET ratios (G) in the cytoplasm (white) or nucleus (grey) in LEDGF/p75KD, LEDGF/p75Flox and HeLaP4 WT cells infected with VSV-G pseudotyped HIVIN-eGFP and HIVIN-mTFP1+IN-mVenus, respectively.
