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A recent report comparing red and white murine skeletal muscles on generic commercial oligonucleotide chips (Affymetrix GeneChips), which comprised the equivalent of 3,000 different genes, yielded a differential list of 49 known genes [ 28].
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Thus, as an alternative, we produced EXIQON microarray of brown and white primary murine adipocytes (prior to and following differentiation) to yield global profiles of miRNAs.
Further, we show that murine white and brown adipocyte cell lines produce profiles that are distinct from each other as well as from precursor cells undergoing differentiation.
Thus, we performed a global expression profiling of miRNAs in white and brown murine primary cell cultures before and after differentiation.
Previous studies (Khan and Tisdale, 1999) have shown propranolol to act as a non-compedative inhibitor of the induction of lipolysis in murine white adipocytes by LMF.
These relate to the finding that the Wdnm1-like transcript is highly enriched in expression in white vs. brown murine adipocytes/adipose tissue -.
A recent study has shown that production of NO, stimulated by caloric restriction, increases SIRT1 expression; this study suggests that eNOS may be involved in regulation of the expression of SIRT1 in murine white adipocytes [ 17].
Induction of lipolysis in murine white adipocytes, and stimulation of adenylate cyclase in adipocyte plasma membranes, by a tumour-produced lipid mobilizing factor, was attenuated by low concentrations (10−7 10−5 M) of the specific β3-adrenoceptor antagonist SR59230A.
From the list of the miRNAs that were differentially expressed during adipogenesis of primary white or brown murine adipocytes, we selected a subset of 10 miRNAs for profiling using Taqman-based qPCR.
The β3-AR mediated coupling of LMF to lipolysis would explain the lowered maximal response of human omental adipocytes to lipolysis when compared with murine white adipocytes (Hirai et al, 1998).
We performed global expression profiling of miRNAs before and after the differentiation of primary murine white and brown preadipocytes into mature adipocytes, in order to discover post-transcriptional regulators of adipogenesis.
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