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The lipid film was hydrated in 10 mM Tris-buffer at pH 7.4, to a final concentration of 5.0 mg/ml DDA and 1.0 mg/ml TDB, by heating for 20 min at 60°C with in-between whirl mixing.
This was done by vacuum concentration for 10 minutes using a Heto Hetovac VR-1 and Heto CT110 vacuum concentrator followed by heating to 60°C for 5 minutes on a water bath and whirl mixing.
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For MBD protein purification E.coli cells (800 μl) were mixed with 500 μl lyses buffer and lysed by repeated bead whirling mixing for 30 s in Lyses Matrix A tubes (MP Biomedicals, Eschwege, Germany) on a FastPrep24 instrument (MP Biomedicals).
Metabolites were dissolved in 0.5 M NaOH, 41.6% (v/v) methanol, 8.4% (v/v) pyridine, and derivatized in silanized glass tubes upon addition of 5% (v/v) methyl chloroformate twice while whirl-mixing for 30 s after each addition [ 28].
Although mixing extraterrestrials with cotton candy, Tilt-A-Whirls and livestock competitions could be conceivably alienating, UFO researcher Mel Podell of the San Diego chapter of the Mutual UFO Network believes it's an out-of-this-world opportunity to raise the profile of Ufology.
He combines the flickering play of blinding light with sudden shafts of darkness, the strut and whirl of bluesy bop mixed with ballads' agonized sentimentality, a craving for simple emotion that gets tangled on the way out.
By mixing these ingredients and melting them together, you create a flammable product that produces whirls of smoke when you light it on fire.
The sample was mixed on a whirl mixer for 10 s and centrifuged for 3 min at 1000 g at 4°C.
For the determination of free camptothecin, 0.25 ml of plasma was added to 0.75 ml 8.5% phosphoric acid and subsequently mixed on a whirl mixer for 10 s.
Very fine bits, which are mixed with a lot more butter and whirled in a mixer until they are as light and fluffy as whipped butter.
Upon arrival, 25 grams of each pooled litter sample was placed into a Whirl-Pak® Filter Bag; 225 mL of BPW was added and mixed for 1 minute.
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