Here, we accounted for the conditions inside the columns as well as to the fact that most analytical instruments are volume-controlled while most preparative scale units are mass-controlled.
Analytical gels were imaged immediately after separation, while the preparative gels were fixed with 7.5% acetic acid/10% methanol overnight and stained with Coomassie Blue R-350.
As a result, while the actual single-molecule experiment consumes typically only a few thousands of molecules, a typical sample preparation requires preparative protein expression, purification and labeling.
From these data we calculated porosities and electrolyte volume fractions of the electrocatalyst layer and agglomerate regions The electrocatalyst layer porosity and electrolyte volume fraction depend directly on the preparative conditions, while the agglomerate porosity and the macropore porosity are not influenced significantly.
The first involved acetylation of a mixture of the three alkaloids and the subsequent purification of cathinone acetate by preparative thin layer chromatography (TLC), while the second made use, after preparative TLC, of fractional crystallization of norephedrine and cathine from a hydrochloric acid solution.
Combining the two models for column pressure and for dynamic binding capacity allows the design of preparative scale columns that maximize productivity while meeting specified pressure constraints.
While DFCCC was carried out on a specially designed preparative (561 ml coil volume) bobbin installed in a similar Midi instrument case.
Fraction 1 gave a mixture of fatty acids (40 mg), while fractions 3 and 4 (80 mg) were further purified by preparative HPLC-DAD to afford 10 mg of abscisic acid (ABA; Figure 1) and 6 mg of its precursor, 1′,4′-trans-diol ABA (diol ABA; Figure 1).
Compared to single column preparative chromatography, SMB separations achieve higher productivity and purity, while reducing the solvent consumption.
In a proportional hazards regression model, the variables that were correlated with an increased probability of cataracts were single-dose TBI (relative risk (RR) = 2.46) and steroid therapy (RR = 2.34), while a decreased probability of cataracts was correlated with a nonTBI preparative regimen (RR = 0.41).
