Northern hybridization analysis showed that HCCA2 mRNA was expressed in 79% (34/43) patients with HCC, most of whom had significantly high expression in HCC tissues, while not expressed in corresponding noncancerous liver tissues.
Estimates of the genome-wide fraction of expressed genes can be inflated by false positives, i.e., genes that, while not expressed, show above background signal intensity because their probes cross-hybridize with the transcript of a closely related gene.
S100A7, while not expressed in normal epithelia, is frequently seen to be expressed in pre-invasive ductal carcinoma in situ [ 48] and is associated with EMT.
For Fhb1 candidate genes discovery, 12 unigenes (indicated by arrows in Figure 8), which were up-regulated in Wangshuibai while not expressed in the mutant, were selected for qRT-PCR validation.
For example, in exocrine cells perhaps the genes characteristic of endocrine cells, while not expressed, are still accessible to transcription factors rather than being sequestered in inactive regions of chromatin.
When administered centrally, MCH causes obesity, while mice not expressing this peptide are lean (49).
Blind larvae responded only to red light while larvae not expressing CsChrimson responded only to blue light.
Intracerebroventribular insulin infusion lowered weight in baboons, with subsequent regain, while animals not expressing insulin receptors in the brain develop obesity (48), supporting this hypothesis (49).
