While Golgi localisation of the endogenous protein and of an EGFP-Irgm1 αK construct apparently identical to our own [20] was reported, no localisation of either to a LAMP1 compartment was reported in uninfected cells.
Interestingly, GFPu accumulation did not occur in neuronal cell bodies in this study, suggesting that while subcellular localisation can affect UPS efficiency in polyglutamine disease, mutant polyglutamine proteins do not necessarily cause general impairment of the UPS.
Nuclear accumulation of β-catenin has recently also been shown in breast cancer (Lin et al, 2000), while nuclear localisation of α-catenin has not been reported.
IL-17 enhanced fra-2 and egr-1 mRNA between T30 T120 with a peak at T90, while nuclear localisation was noticed at T30 for Fra-1, JunD and NF-κB.
Furthermore, while nuclear localisation was associated with good prognostic features (being related to better differentiation, low angiogenesis, and negative lymph node status), cytoplasmic and combined nuclear/cytoplasmic localisation were associated with poor prognostic factors, such as angiogenesis together with node and p53 positivity (Kakolyris et al, 1998; Puglisi et al, 2002).
Intriguingly, while the initial localisation was not silencing-dependent, the spread to actively transcribed regions required a transcriptionally competent Xist RNA [ 39].
Our results clearly demonstrate that within a given sample, we were more likely to find nuclear RelA and RelB, while the nuclear localisation of RelA and p50 was less frequently observed.
The positivity for the antigens ODC, PUT, and SPM had a cytosolic paranuclear localisation, while for the DAO antigen a basolateral positivity was appreciable according to what was observed by Oliva et al. [ 6].
A striking difference between ISP1-GFP and ISP3-GFP was observed in ookinetes: while ISP1-GFP localisation was strong and largely limited to the apical tip of the ookinete, ISP3-GFP was distributed along the parasite periphery.
