We conclude that MK2 is involved in maintaining bipolar microtubule organization while microtubule disassembly does not affect MK2 function on chromosomes.
Beyond his work at Berkman Klein, David spends his free time pretending to be a chef at home, occasionally voiding warranties while doing DIY disassembly and repair, and spending as much time as possible outdoors in Boston, California, and Colorado.
We studied buffer conditions that maximized this disassembly while still allowing robust assembly to take place.
GMPPNP, GMPPCP, and ATP could each enhance the rate of assembly and stabilize the plateau of assembled microtubules against disassembly, while not binding appreciably to free tubulin.
The αB crystallin interactive sequences 131LTITSSLSSDGV142 and 156ERTIPITRE164 promote microtubule assembly and inhibit microtubule disassembly, while the interactive sequence 113FISREFHR120 inhibited both microtubule assembly and disassembly.
It has been reported that nocodazole and colchicine block the assembly of centrioles/basal bodies while not causing disassembly of existing ones (Boisvieux-Ulrich et al., 1989a; Jensen et al., 1987; Balczon et al., 1999; Le Clech, 2008).
In a working model, Sec17 and SMs accelerate fusion mediated by cognate SNARE complexes and protect them from NSF-mediated disassembly, while mis-assembled or non-cognate SNARE complexes are eliminated through kinetic proofreading by Sec18.
These findings led to proposals that SMs protect pre-fusion SNARE complexes from premature disassembly while exposing post-fusion complexes and mis-assembled or non-cognate pre-fusion complexes to disassembly by Sec18 (Mima et al., 2008; Starai et al., 2008; Rizo and Südhof, 2012).
Case-based reasoning method is used to determine recycling strategy and performance of disassembly operation while economic analysis model is used for quick estimating recycling cost and benefit.
