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After amplification, melting curve analysis was performed by heating the PCR products to 95°C for 15 sec, then cooling it to 60°C for 15 sec, followed by a linear temperature increase to 95°C at a rate of 0.3°C/sec while continuously monitoring the fluorescent signal.
"But in the face of the critical challenge in West Africa, the WHO and Western medical agencies should be helping countries weigh the risks and benefits of limited deployment of the best (drug and vaccine) candidates to those in the greatest need, while continuously monitoring safety and efficacy".
Thereafter, the lungs were placed in a plastic bag (Vitrolife) containing Perfadex® solution and stored on ice for 8 h while continuously monitoring lung surface temperature (Medical Temperature Probes, Siemens S.p.A., Milan, Italy).
A series of sandwich specimens with and without embedded piezoelectric sensor were tested in three-point bending in static and fatigue loading while continuously monitoring the response by the AE technique.
A similar protocol was repeated using MS222 (10-6 M to 10-3 M) while continuously monitoring heart rate and ventricular wall motion.
Stepped care is based on treating patients with the most adequate treatment of lowest intensity while continuously monitoring their treatment progress.
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"We're continuously monitoring water quality," Mr. Sheehan said.
To test this prediction of the Dbx1 core hypothesis, we used photonics to map the positions of Dbx1 preBötC neurons, and then laser ablated them one cell at a time while continuously monitoring respiratory motor output (Wang et al., 2013).
However, while we continuously monitor the situation, we believe we have positioned the portfolio well to deal with these pressures.
The specimens were cast using reactive aggregate and mortar with a high alkali content and placed in a controlled environment with high humidity and temperature to accelerate the reaction, while being continuously monitored with acoustic emission.
In wireless sensor networks, querying services purpose to detect static events while tracking services continuously monitor mobile events.
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