Related to aggregation, the Radiator also introduces the concept of delayed propagation, based on the principle that some context messages may be temporarily retained before being propagated while still fulfilling user expectations.
While we bear responsibility for our problems, the caricature of Mexico being propagated in the United States only increases the despair on both sides of the Rio Grande.
In a typical experiment, of 629 total colonies obtained prior to the first passage, we monitored 24 that were clonally derived from single cells and, of these, 12 were frozen as before passage while 4 were propagated as distinct lines.
Maintenance and manipulation of all strains of C. elegans have been previously described (13 18) while CL4176 was propagated at 16°C on solid nematode growth medium (NGM) seeded with 100 µl spots of E. coli (OP50) for food.
All WT strains were grown on Vero cells while mutant viruses were propagated on U2OS cells with 3 mM HMBA.
E. coli strains were grown in Luria-Bertani (LB; Invitrogen) broth or on LB agar plates while S. flexneri was propagated either in LB broth, tryptic soy broth (TCS; Sigma) or on TCS plates with Congo red (0.01%, Sigma).
In a (meth)acrylate chain-growth reaction, a vinyl carbon radical is propagated, while in the thiol ene step-growth mechanism, each propagation step results in both consumption and regeneration of a thiyl radical.
While human MECs can be propagated for a limited number of times, mouse MECs behave differently and do not proliferate well after the first passage.
Using defined media that includes the presence of either an inhibitor of FGF signaling or its downstream Erk/MAP kinase transduction pathway, mouse ESCs (mESCs) can be propagated while maintaining the expression of these pluripotency markers (Ying et al., 2008).
While susceptibility to chronic infection is propagated by T helper cell type 1 cytokine responses (characterised by production of IL-12, IL-18 and interferon-γ), immunity to intestinal-dwelling adult nematode worms is critically dependent on a type 2 cytokine response (controlled by CD4+T helper type 2 cells that secrete the cytokines IL-4, IL-5, IL-9 and IL-13).
Less robust plants, including agave (pictured) and Dracaena draco, will spend the winter in a polytunnel while salvias and other tender perennials are propagated from cuttings in September.
