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From each isolated Caenorhabditis sample, we established cultures, which were cryopreserved and stocked in our collections.
Leukapheresis yielded more than 1 × 10 PBMC and 100 mL plasma, both of which were cryopreserved until use.
Inter-assay variability was determined for both MFI and the percentage of ζ-chain-negative cells, using cells of one normal donor which were cryopreserved, thawed and tested for ζ-chain expression every time the assay was performed on 10 different days.
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Establishing a biobank of allogeneic genetically modified immune cells from healthy third-party donors, which are cryopreserved and validated in advance of administration, will facilitate the centralizing manufacturing and widespread distribution of CAR+T-cells to multiple points-of-care in a timely manner.
The Taiaçu strain of R. rickettsii used in this experiment was the first guinea pig passage from a naturally infected A. aureolatum tick, which was cryopreserved before this strain was adapted for in vitro growth (5 ).
Briefly, this involved maintaining N2 × DR1350 F1 cross progeny, produced from both N2 hermaphrodite by DR1350 male crosses and reciprocal matings, individually and allowing them to self-fertilise for at least 30 generations, at which point they were cryopreserved.
Among them, a cell specimen, a part of which had been cryopreserved as living cells, was included.
Thus, the cells were cryopreserved from 4th to 6th passages.
Half of the cells were cryopreserved for 3 months.
Freshly-isolated RC were cryopreserved.
AC pellets were cryopreserved in liquid nitrogen.
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