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Cells, which were acclimatized for 30 cycles, were then tested for butanol tolerance (up to 2.25%v/v).
According to the body weight (approximately 30 g), they were randomly divided into different groups of 5-6 animals which were acclimatized for a week before starting the experiment.
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Four groups of five rats each were acclimatized on pelletized mouse chow for seven days, after which diabetes was induced by a single ip injection of alloxan in all groups except group 1, which served as control.
They were acclimatized to the animal house condition for 7 days during which they were allowed free access to commercial pelleted rat chow (Pioneer Food (Pty) Ltd, Huguenot, South Africa) and water.
Thus, interspecific variation in BMR reflects in part the conditions to which birds are acclimatized or acclimated at the time of metabolic measurements, and not necessarily genotypic divergence.
Rats were acclimatized for 5 days prior to use, in a room in which a suitable environment was maintained.
Before field planting, tissue-culture plants were acclimatized in a secure shade house over a 3-month period by which time they had reached a height of ~35 cm.
The rats were acclimatized at least for one week before experiments in an animal facility in which the appropriate temperature, humidity, and light cycle (6:00 A.M.–6:00 P.M) were maintained, with ad libitum access to food and water.
Age (21 days) and weight matched female mice (n = 140) were acclimatized to a 12 h day: 12 h night cycle for 3 wk, during which time they were fed the control diet.
Upon arrival at the Mini Hatchery Complex, fishes were acclimatized, separated into male and female and kept in four cisterns (1.23 × 2.44 × 0.46 m) of which two cisterns were used for treatment and two for control.
Plantlets were acclimatized under glass house conditions with 90% survival.
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