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Figure 1A shows the SV40-based episome (shuttle vector) (Parris and Seidman, 1992) into which we inserted a mismatch region (MM1).
The APOA-II gene is expressed in liver and intestine, and we previously constructed a lentivector in which we inserted APOA-II regulatory sequences to drive GFP expression, and confirmed its functionality both in vitro and in vivo[ 12, 13].
To support our RNA-Seq analysis data and to investigate the expression and localization of fibrillar and tubular muscle-specific genes or gene isoforms, we generated a number of genomic fosmid reporter transgenes 11 in which we inserted a GFP tag into the protein or protein isoform of interest by recombineering 12.
We first tested the influence of miR-9, miR-17-5p, miR-146a, miR-146b-5p miR-146b-5p miR-146b-5pmessenger target by using a repontheirector into which we inserted the entire 3′UTR of BRCA1 downstream of the firefly luciferase opredictedng framessenger
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Our cars and homes are computers into which we insert our bodies; our hearing aids and implanted defibrillators are computers we insert into our bodies.
Take the Boston Gazette, from whose pages we freely adapt a number of news items, and into which we insert letters, editorials, and advertisements of our own devising.
To finish the proof we need the following inequality which is the direct consequence of (58): int_{0}^{1}theta^{2}v^{2},dx leq C_{8}epsilonint_{0}^{1}rho4} rho biggl(frac {partialtheta}{partial x} biggr)^{2},dx+C_{epsilon}biggl(1+ int_{0}^{t}int_{0}^{1} rho r^{4} biggl(frac{partialtheta}{partial x} biggr)^{2},dx,dtau biggr) (85) which we insert into (84) and use the suitable ϵ.
Let us proceed to the last term of Equation (25), in which we insert Equation (22) and consequently the equality ĥ p, i = h p, i − n est, i, which states that the channel estimate at the pilot symbol position is given as the true channel superimposed by an estimation error.
Given the almost universal acceptance at the time of the necessity of deregulation, that's also an intriguing area in which we need to insert ourselves.
pTL13 is an RNAi construct targeting unc-43, which we generated by inserting a 332-bp cDNA fragment of unc-43 into the multiple cloning sites of pAD12.
For identification of cells in which recombination had occurred, we inserted an internal ribosomal entry-enhanced green fluorescent protein (IRES-GFP) cassette downstream of Sox2.
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CEO of Professional Science Editing for Scientists @ prosciediting.com