The fusion protein eluted as a single major peak which was concentrated using Vivaspin concentrators (GE Healthcare), and buffer-exchanged into 50 mM Tris-Cl, pH 7.5.
The fusion protein eluted as a single major peak (spanning several fractions) which was concentrated using Vivaspin concentrators (GE Healthcare), and buffer-exchanged into 20 mM Tris-Cl, pH 7.5, 300 mM NaCl.
Purification of TAP-tagged complexes was carried out as previously described [ 25] with the following modifications: Elutions were carried out in a volume of 500 μl and repeated five times for a total volume of 3 ml of purified complexes, which was concentrated six-fold with Amicon Ultra concentrators with a MW cutoff of 100 kilodaltons.
Similarly, co-crystallization with cAMP was accomplished by adding cAMP to a final concentration of 5 mM to the protein sample, which was concentrated with a 10 kDa cutoff Amicon Ultra (Millipore) to 17 mg/ml.
The scientists had better images for the analysis of Mr. Trump's inauguration crowd, which was concentrated on the mall.
Southern Democrats stoutly supported the Truman Administration's military buildup, much of which was concentrated in the South.
Last year investors the world over were eager to invest in the so-called new economy, which was concentrated in this country.
The corporation was responsible for overseeing the state's $8.6 billion school building program, which was concentrated in the state's poorest districts.
The honor of destroying this set of values fell to that part of the American left which was concentrated spiritually around the Berkley campus in mid-nineteen-sixties.
Hospital transmissions were a key factor a decade ago in the outbreak of SARS — severe acute respiratory syndrome — which was concentrated in Asia and caused hundreds of deaths.
They claimed rights in bishops' churches and monasteries far outside their immediate domain, which was concentrated around Paris, Orléans, Compiègne, Soissons, and Beauvais.
