First, an extra copy of the PII biosynthetic gene cluster, which was assembled using a modified Gibson assembly method for cloning large DNA fragments with high GC contents, was introduced into a high-producing strain S. pristinaespiralis HCCB10218.
Comparing with the previously released A. mediterranei S699 assembly [ 32], which was assembled using A. mediterranei U32 as a reference, the genome we sequenced contained a 10-kb insertion.
The volume fraction of the solution is around 2.0 wt% PSS which was assembled onto the silicon and glass substrates by horizontal self-assembly method.
The total assembly size of the genome of S. scitamineum is 19.8 Mb which was assembled into 321 contigs and 58 scaffolds (Table 1).
The collection, which was assembled over a period of 70 yrs.
Initially, he was hesitant to get involved with the defense project, which was assembled by the A.C.L.U.
The archdiocese hired a calligrapher to print the names in the book, which was assembled just for the pope, Father Connolly said.
The cable operation, which was assembled through AT&T's acquisitions of Tele-Communications and the MediaOne Group, was to be spun off in 2002.
Five thousand men spent three years building the ship, which was assembled at the Harland and Wolff shipyard on Queen's Island.
Mr. Paterson's embrace of Wall Street was, naturally, well received by his audience, which was assembled a block east of the New York Stock Exchange.
This date is not mentioned, however, in 2666, which was assembled from Bolaño's files under the supervision of his literary executor Ignacio Echevarría, a Spanish critic.
