The large number of statistical tests carried out creates the possibility that findings could be false positives and as this was a pilot and feasibility study which, therefore, used a low power we could potentially fail to detect small to moderate sized effects.
Furthermore, in a follow-up study in older children (which therefore used the operant conditioning train paradigm), acquisition of the task occurred when infants were 6-months-old, followed by five spaced reminders in the intervening period (with the final reminder occurring at 18-months of age).
Pre-tests showed that plant growth was not impaired at 22-g fresh manure/kg dry soil, which therefore was used for the final tests.
The model-error is also minimal for this perturbation function, which therefore was used in the final model.
Successful transfection of siRNA and expression vectors was confirmed in this cell line, which therefore was used as negative control in subsequent experiments (Supporting Information Fig S1B).
For baseline neutrophils, the median value (5.46) was close to the predefined cutoff level of 6.0 × 10 LopezLopet et al, 1996), which therefore was used as cutoff for the analyses.
Boron nitride is insoluble in usual acids, but is soluble in alkaline molten salts and nitrides, such as LiOH, KOH, NaOH-Na2CO3, NaNO3, Li3N, Mg3N2, Sr3N2, Ba3N2 or Li3BN2, which are therefore used to etch BN.
Human insulin was stocked in HEPES buffer, which was therefore used in controls for all the experiments with insulin treatment.
The largest and most reliable FRET ratio changes were observed with the D157N-based reporter, which was therefore used for all subsequent experiments.
In four studies PA energy expenditure was reported and used as the exposure in the meta-analysis [22], [23], [25], [26]; one study only reported results of interest on total energy expenditure, which was therefore used [21].
Substantial inhibition was seen at 5 × 10−6 M Gefitinib, which was therefore used in subsequent experiments.
