IVPR cells and iNANOG cells were cultured in 2iL medium which supplemented with ERK inhibitor PD0325901, GSK3 inhibitor CHIR99021, human LIF, and bFGF proteins with or without Dox addition (Silva et al., 2009; Takashima et al., 2014).
Correspondingly, we point at a severely impaired thalamic dopaminergic neurotransmission which, supplemented with a deficient nigrostriatal dopaminergic innervation, might mediate non-parkinsonian symptoms, such as depression, in DLB as well.
Fresh oral and rectal specimens were handled and inoculated directly on Sabouraud dextrose agar plates (SDA, Oxoid, Ltd, Basingstoke, UK) which supplemented with chloramphenicol (0.05 g/l) and incubated at 37°C for 24-48 hrs.
For the extraction of free DNA, we used 200 μl of stored serum, which supplemented with 1 μg salmon testes DNA (Sigma, St Louis, MO, USA) as a carrier DNA.
Håkons Hall Sports Centre is part of the complex which supplements the main hall with a recreational training facility.
The threat of the Z.E.V. mandate, as it is known in the trade, spurred the development of hybrid vehicles, which supplement gasoline with electric power.
Even in restaurants like Giorgione 508 in SoHo, which supplement their counters with a few tables, the counter is clearly the place to be.
From the Obama campaign, which supplemented traditional polling methods with advanced data analytics drawn from public voting records, the criticism was more pointed.
He goes on field trips, taking along canned meats which he supplements with wild plants & fruits.
His piano playing, which he supplements with sporadic obbligatos on the kazoo, is compelling, though hardly virtuosic.
Mr. Mercado creates and wears an elaborate wardrobe of gowns, veils and capes, which he supplements with wigs, extravagant makeup and portable props (pineapples, dolls, gilded picture frames).
