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The AOPP assay includes a sample preparation procedure to precipitate triglicerides (3.000 g for 10 minutes in the presence of 25 mM/L MgCl2 and 0.5 mM/L phosphotungstic acid) which strongly interfere with the determination of the marker [ 14].
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In our preliminary experiments we found that after 2.5 h incubations both S. Typhimurium bacteria and zymosan particles give high background fluorescence, which strongly interfered with fluorescence of Annexin V-Alexa Fluor 488 Conjugate, and just low background with Annexin V-Alexa Fluor 647, so we used the latter to stain phosphatidylserine expression on apoptotic cells.
"A financially devastated and chaotic Mexico would strongly interfere with our ability to maintain an East-West balance," he wrote.
Moreover, mutations at A2971 strongly interfere with peptide release.
In addition, JH III and JH agonist (JHA) strongly interfere with this 20E-dependent immune competence.
Despite a high sensitivity, the mutant discrimination ratio here is relatively moderate compared to other specific SNP assays, which may limit its potential for applications in real clinical samples where the presence of large background genomic DNAs and/or wild-type genes may strongly interfere the specific SNP detection.
Adding synthetic floral scents to infested vegetative plants made them considerably less attractive to parasitoids, which strongly suggests that floral volatiles do in fact negatively interfere with attractive HIPVs in the headspace and contribute to making infested flowering plants less attractive to C. glomerata wasps.
Additionally, this molecule also interferes with OPG/RANKL/RANK cytokine system, essential for osteoclast biology, which strongly suggests that this SERM can modulate osteoclast activity by a paracrine osteoblast-dependent mechanism (Hofbauer et al 2004).
We identify four highly conserved promoter elements, each of which is dispensable for BB expression; however, the combined loss of some or all of these elements strongly interferes with BB promoter activity.
The TDMA ST scheme schedules the weakly interfering links to transmit simultaneously, and schedules the strongly interfering links to transmit at different time slots.
The gels are also used to shut off the flow of water that strongly interferes with hydrocarbon production and substantially reduces the profitability of wells.
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