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To determine if LZ+ MYPT1 expression was modulated in heart failure, we used Western blotting with two different antibodies, one of which recognized all MYPT1 isoforms and an anti-LZ+ MYPT1 antibody.
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BMP-2 was still able to induce activation of other p38 proteins, visualized by immunoblot against phosphorylated p38 which recognizes all phosphorylated p38 proteins.
Thus, we determined the presence of leukocytes in the RPE/choroid by using CD45, which recognizes all leukocytes including granulocytes, lymphocytes and macrophages.
In eukaryotes, this process is mediated by two release factors: eRF1 which recognizes all three stop codons and triggers hydrolysis of the final peptidyl-tRNA bond, and eRF3 which stimulates eRF1 activity in a GTP-dependent manner [1].
Individual substitutions for G106 or L107 residues reduced the reactivities predominately against group-cross-reactive MAbs which recognize all flavivirus E proteins (Tables 1 and 2, some data not shown).
Immunohistochemistry with our PA3111 antibody [16], [43] (Figures 1B, C and 2), which recognizes all three major isoforms of dysbindin-1, revealed that this protein is neuronal, not glial.
Cells were processed for immunostaining as described before [11] except that the primary antibody solution consisted of a 1∶50 dilution of murine monoclonal antibody 303.9 (PROGEN Biotechnik; catalog number: 61069), which recognizes all four AAV Rep proteins.
We also used antibody, which recognizes all the three isoforms of ShcA family of proteins.
These fibers were also labeled by BF-35 antibody, which recognizes all MyHC isoforms, except -2x.
Antibody LM137, which recognizes all H determinants and Leb tetrasaccharide, also labeled connective tissue and digestive epithelial cells intracellularly.
High levels of colocalization were seen in sections colabeled with αAPF and Tau-5, which recognizes all forms of tau.
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