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The majority of control AR42J-B13 co-expressedressed the exocrine marker amylase and CK20, which labels both progenitor-like and ductal cells [22].
Similarly, anti-NFC2 which labels both neurofascin 155 (NF 155) proteins at paranodes and neurofascin 186 (NF 186) proteins at nodes (Fig. 4C) diffused from paranodes to juxtaparanodes.
50, 51 On the other hand, I-salmon calcitonin, which labels both calcitonin and amylin-binding sites, 52 shows binding in this region of rat and monkey brain.
To do this, we utilised the lyve1 DsRed2 kdrl EGFP compound transgenic line which labels both the lymphatic/venous (lyve1) and blood (kdrl) vasculature and can therefore be used to differentiate the lymphatic vessels from the blood vessels (Okuda et al., 2012).
Isolectin B4 (IB4) staining, which labels both blood vessels and microglia, highlighted the amoeboid morphology of activated microglia around the hemorrhage site in the PDGFrβ-cre;Foxc1fl-fl cortex (Fig. 3G); activated microglia are characteristic of an inflammatory response, presumably caused by the hemorrhage.
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The respective microsomal fractions were immunoprecipitated with anti-CN-A antibody, run on SDS-PAGE and analyzed by Western blot with anti-PERK antibody, which labeled both phosphorylated and the higher mobile unphosphorylated PERK.
The use of two different epithelial-specific antibodies, against EpCam and cytokeratin 7/8, makes it most probable that cells, which label with both antibodies, are indeed CTCs.
However, in mutant embryos, expression of both vsx2, which labels V2a interneurons (6), and vsx1, which labels V2a/b interneuron precursors (7), was significantly decreased throughout the CNS (Figs 4E,F and 5A D).
Shown are confocal images of nuclei stained with LacI::GFP, which labels lacO arrays integrated into both chromosome V homologs (top panels) and antibodies to the axial/lateral element protein HTP-3 and the central region protein SYP-1 (bottom panels) (A ) SC proteins associate with pachytene chromosomes of wild-type animals, rec-8 and spo-11 single mutants, and spo-11 rec-8 double mutants.
The second version of the barcoding method, which involved labelling both strands with the same barcode sequence, did not improve the accuracy compared with that of the single strand labelling (Fig. 4).
However, development of [3H]hemicholinium-3 binding which labels the presynaptic high affinity cholinergic transporter, was deficient in both striatum and hippocampus.
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